Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant

© 2020 The Author(s)..

Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%-50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8-92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity.

Media Type:

Electronic Article

Year of Publication:

2020

Contained In:

Cell reports. Medicine - Vol. 1, No. 6 (2020), p. 100099

Language:

English

Contributors:

Mlcochova, Petra
Collier, Dami
Ritchie, Allyson
Assennato, Sonny M
Hosmillo, Myra
Goel, Neha
Meng, Bo
Chatterjee, Krishna
Mendoza, Vivien
Temperton, Nigel
Kiss, Leo
James, Leo C
Ciazynska, Katarzyna A
Xiong, Xiaoli
Briggs, John A G
Nathan, James A
Mescia, Federica
Bergamaschi, Laura
Zhang, Hongyi
Barmpounakis, Petros
Demeris, Nikos
Skells, Richard
Lyons, Paul A
Bradley, John
Baker, Steven
Allain, Jean Pierre
Smith, Kenneth G C
Bousfield, Rachel
Wilson, Michael
Sparkes, Dominic
Amoroso, Glenn
Gkrania-Klotsas, Effrosyni
Hardwick, Susie
Boyle, Adrian
Goodfellow, Ian
Gupta, Ravindra K
Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration
Baker, Stephen
Bradley, John
Dougan, Gordon
Goodfellow, Ian
Gupta, Ravi
Lehner, Paul J
Lyons, Paul
Matheson, Nicholas J
Smith, Kenneth Gc
Toshner, Mark
Weekes, Michael P
Brown, Nick
Curran, Martin
Palmar, Surendra
Zhang, Hongyi
Enoch, David
Chapman, Daniel
Shaw, Ashley
Jose, Sherly
Bermperi, Areti
Zerrudo, Julie Ann
Kourampa, Evgenia
Watson, Laura
Worsley, Jieniean
Saunders, Caroline
de Jesus, Ranalie
Domingo, Jason
Pasquale, Ciro
Vergese, Bensi
Vargas, Phoebe
Fabiculana, Marivic
Perales, Marlyn
Mynott, Lee
Blake, Elizabeth
Bates, Amy
Vallier, Anne-Laure
Williams, Alexandra
Phillips, David
Chiu, Edmund
Overhill, Alex
Ramenatte, Nicola
Sipple, Jamal
Frost, Steven
Knock, Helena
Hardy, Richard
Foster, Emily
Davidson, Fiona
Rundell, Viona
Bundi, Purity
Abeseabe, Richmond
Clark, Sarah
Vicente, Isabel
Watson, Laura
Worsley, Jieniean
Elmer, Anne
Ribeiro, Carla
Kourampa, Jenny
Jose, Sherly
Kennet, Jane
Rowlands, Jane
Meadows, Anne
O'Brien, Criona
Rastall, Rebecca
Crucusio, Cherry
Hewitt, Sarah
Price, Jane
Calder, Jo
Canna, Laura
Bucke, Ashlea
Tordesillas, Hugo
Harris, Julie
Ruffolo, Valentina
Domingo, Jason
Graves, Barbara
Butcher, Helen
Caputo, Daniela
Le Gresley, Emma
Dunmore, Benjamin J
Martin, Jennifer
Legchenko, Ekaterina
Treacy, Carmen
Huang, Christopher
Wood, Jennifer
Sutcliffe, Rachel
Hodgson, Josh
Shih, Joy
Graf, Stefan
Tong, Zhen
Mescia, Federica
Tilly, Tobias
O'Donnell, Ciara
Hunter, Kelvin
Pointon, Linda
Pond, Nicole
Wylot, Marta
Jones, Emma
Fawke, Stuart
Bullman, Ben
Turner, Lori
Jarvis, Isobel
Omarjee, Ommar
De Sa, Aloka
Marsden, Joe
Betancourt, Ariana
Perera, Marianne
Epping, Maddie
Richoz, Nathan
Bower, Georgie
Sharma, Rahul
Nice, Francesca
Huhn, Oisin
Fawke, Stuart
Savoinykh Yarkoni, Natalia
Romashova, Nika
Lewis, Daniel
Hinch, Andrew
Cossetti, Chiara
Strezlecki, Mateusz
Grenfell, Richard
Stark, Hannah
Walker, Neil
Stirrups, Kathy
Ovington, Nigel
Dewhust, Eleanor
Li, Emily
Papadia, Sofia
Kingston, Nathalie
Lever, Andrew
Torok, Estee
Hamilton, William
Hall, Grant
Jahun, Aminu
Chaudhry, Yasmin
Pinckert, Malte
Georgana, Iliana
Yakovleva, Anna
Caller, Laura
Caddy, Sarah
Feltwell, Theresa
Khokhar, Fahad
Meredith, Luke
Holdcroft, Charlotte
Curran, Martin
Parmar, Surendra
Kingston, Nathalie
Lever, Andrew
Torok, Estee
Hamilton, William
Hall, Grant
Jahun, Aminu
Chaudhry, Yasmin
Pinckert, Malte
Georgana, Iliana
Yakovleva, Anna
Caller, Laura
Caddy, Sarah
Feltwell, Theresa
Khokhar, Fahad
Meredith, Luke
Holdcroft, Charlotte
Curran, Martin
Parmar, Surendra

Links:

Volltext

Keywords:

COVID-19
D614G
Journal Article
Point of care testing
Rapid diagnoses
SARS-CoV-2

Notes:

Date Revised 21.11.2020

published: Print-Electronic

Citation Status PubMed-not-MEDLINE

Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Physical Description:

Online-Ressource

doi:

10.1016/j.xcrm.2020.100099

PMID:

32905045

PPN (Catalogue-ID):

NLM315725206