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A thermostable, closed SARS-CoV-2 spike protein trimer

The spike (S) protein of SARS-CoV-2 mediates receptor binding and cell entry and is the dominant target of the immune system. It exhibits substantial conformational flexibility. It transitions from closed to open conformations to expose its receptor-binding site and, subsequently, from prefusion to postfusion conformations to mediate fusion of viral and cellular membranes. S-protein derivatives are components of vaccine candidates and diagnostic assays, as well as tools for research into the biology and immunology of SARS-CoV-2. Here we have designed mutations in S that allow the production of thermostable, disulfide-bonded S-protein trimers that are trapped in the closed, prefusion state. Structures of the disulfide-stabilized and non-disulfide-stabilized proteins reveal distinct closed and locked conformations of the S trimer. We demonstrate that the designed, thermostable, closed S trimer can be used in serological assays. This protein has potential applications as a reagent for serology, virology and as an immunogen

Year of Publication: 2020
Contained in: Nature structural & molecular biology Vol. 27, No. 10 (2020), p. 934-941
All journal articles: Search for all articles in this journal
Language: English
Contributors: Xiong, Xiaoli | Author
Qu, Kun
Ciazynska, Katarzyna A
Hosmillo, Myra
Carter, Andrew P
Ebrahimi, Soraya
Ke, Zunlong
Scheres, Sjors H W
Bergamaschi, Laura
Grice, Guinevere L
Zhang, Ying
CITIID-NIHR COVID-19 BioResource Collaboration
Nathan, James A
Baker, Stephen
James, Leo C
Baxendale, Helen E
Goodfellow, Ian
Doffinger, Rainer
Briggs, John A G
Bradley, John
Lyons, Paul A
Smith, Kenneth G C
Toshner, Mark
Elmer, Anne
Ribeiro, Carla
Kourampa, Jenny
Jose, Sherly
Kennet, Jane
Rowlands, Jane
Meadows, Anne
O'Brien, Criona
Rastall, Rebecca
Crucusio, Cherry
Hewitt, Sarah
Price, Jane
Calder, Jo
Canna, Laura
Bucke, Ashlea
Tordesillas, Hugo
Harris, Julie
Ruffolo, Valentina
Domingo, Jason
Graves, Barbara
Butcher, Helen
Caputo, Daniela
Le Gresley, Emma
Dunmore, Benjamin J
Martin, Jennifer
Legchenko, Ekaterina
Treacy, Carmen
Huang, Christopher
Wood, Jennifer
Sutcliffe, Rachel
Hodgson, Josh
Shih, Joy
Graf, Stefan
Tong, Zhen
Mescia, Federica
Tilly, Tobias
O'Donnell, Ciara
Hunter, Kelvin
Pointon, Linda
Pond, Nicole
Wylot, Marta
Jones, Emma
Fawke, Stuart
Bullman, Ben
Bergamaschi, Laura
Turner, Lori
Jarvis, Isobel
Omarjee, Ommar
De Sa, Aloka
Marsden, Joe
Betancourt, Ariana
Perera, Marianne
Epping, Maddie
Richoz, Nathan
Bower, Georgie
Sharma, Rahul
Nice, Francesca
Huhn, Oisin
Stark, Hannah
Walker, Neil
Stirrups, Kathy
Ovington, Nigel
Dewhust, Eleanor
Li, Emily
Papadia, Sofia
Full text access:
Electronic availability is being checked...
Links: Full Text (dx.doi.org)
Keywords: Journal Article
Research Support, Non-U.S. Gov't
ISSN: 1545-9985
Note: Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Notes: Date Revised 21.10.2020
published: Print-Electronic
Citation Status In-Process
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PMID:
    32737467
Physical Description: Online-Ressource
ID (e.g. DOI, URN): 10.1038/s41594-020-0478-5
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700 1 |a Ke, Zunlong 
700 1 |a Scheres, Sjors H W 
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700 1 |a Grice, Guinevere L 
700 1 |a Zhang, Ying 
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700 1 |a Jose, Sherly 
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700 1 |a Rowlands, Jane 
700 1 |a Meadows, Anne 
700 1 |a O'Brien, Criona 
700 1 |a Rastall, Rebecca 
700 1 |a Crucusio, Cherry 
700 1 |a Hewitt, Sarah 
700 1 |a Price, Jane 
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700 1 |a Canna, Laura 
700 1 |a Bucke, Ashlea 
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700 1 |a Harris, Julie 
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700 1 |a Bower, Georgie 
700 1 |a Sharma, Rahul 
700 1 |a Nice, Francesca 
700 1 |a Huhn, Oisin 
700 1 |a Stark, Hannah 
700 1 |a Walker, Neil 
700 1 |a Stirrups, Kathy 
700 1 |a Ovington, Nigel 
700 1 |a Dewhust, Eleanor 
700 1 |a Li, Emily 
700 1 |a Papadia, Sofia 
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