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Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV

Middle East respiratory syndrome coronavirus (MERS-CoV) is a WHO priority pathogen for which vaccines are urgently needed. Using an immune-focusing approach, we created self-assembling particles multivalently displaying critical regions of the MERS-CoV spike protein ─fusion peptide, heptad repeat 2, and receptor binding domain (RBD) ─ and tested their immunogenicity and protective capacity in rabbits. Using a "plug-and-display" SpyTag/SpyCatcher system, we coupled RBD to lumazine synthase (LS) particles producing multimeric RBD-presenting particles (RBD-LS). RBD-LS vaccination induced antibody responses of high magnitude and quality (avidity, MERS-CoV neutralizing capacity, and mucosal immunity) with cross-clade neutralization. The antibody responses were associated with blocking viral replication and upper and lower respiratory tract protection against MERS-CoV infection in rabbits. This arrayed multivalent presentation of the viral RBD using the antigen-SpyTag/LS-SpyCatcher is a promising MERS-CoV vaccine candidate and this platform may be applied for the rapid development of vaccines against other emerging viruses such as SARS-CoV-2

Year of Publication: 2020
Contained in: Emerging microbes & infections Vol. 9, No. 1 (2020), p. 1080-1091
All journal articles: Search for all articles in this journal
Language: English
Contributors: Okba, Nisreen M A | Author
Widjaja, Ivy
van Dieren, Brenda
Aebischer, Andrea
van Amerongen, Geert
de Waal, Leon
Stittelaar, Koert J
Schipper, Debby
Martina, Byron
van den Brand, Judith M A
Beer, Martin
Bosch, Berend-Jan
Haagmans, Bart L
Full text access:
Electronic availability is being checked...
Links: Full Text (dx.doi.org)
Keywords: Journal Article
MERS-coronavirus
SARS-CoV-2
Vaccine
i301
lumazine synthase
rabbit
spike
spytag-spycatcher
Additional Keywords: *Antibody Formation
*Antigen Presentation
Animals
Antibodies, Neutralizing
Antibodies, Viral
Antibody Affinity
Binding Sites
Coronavirus Infections
Enzyme-Linked Immunosorbent Assay
Female
Genetic Vectors
HEK293 Cells
Humans
Immunogenicity, Vaccine
Middle East Respiratory Syndrome Coronavirus
Neutralization Tests
Protein Binding
Protein Domains
Rabbits
Spike Glycoprotein, Coronavirus
Viral Vaccines
Virus Replication
ISSN: 2222-1751
Note: Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Notes: Date Completed 02.06.2020
Date Revised 02.06.2020
published: Print
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PMID:
    32471334
Physical Description: Online-Ressource
ID (e.g. DOI, URN): 10.1080/22221751.2020.1760735
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