Differential immunohistochemical expression profiles of perlecan-binding growth factors in epithelial dysplasia, carcinoma in situ, and squamous cell carcinoma of the oral mucosa

Copyright © 2016 Elsevier GmbH. All rights reserved..

The intercellular deposit of perlecan, a basement-membrane type heparan sulfate proteoglycan, is considered to function as a growth factor reservoir and is enhanced in oral epithelial dysplasia and carcinoma in situ (CIS). However, it remains unknown which types of growth factors function in these perlecan-enriched epithelial conditions. The aim of this study was to determine immunohistochemically which growth factors were associated with perlecan in normal oral epithelia and in different epithelial lesions from dysplasia and CIS to squamous cell carcinoma (SCC). Eighty-one surgical tissue specimens of oral SCC containing different precancerous stages, along with ten of normal mucosa, were examined by immunohistochemistry for growth factors. In normal epithelia, perlecan and growth factors were not definitely expressed. In epithelial dysplasia, VEGF, SHH, KGF, Flt-1, and Flk-1were localized in the lower half of rete ridges (in concordance with perlecan, 33-100%), in which Ki-67 positive cells were densely packed. In CIS, perlecan and those growth factors/receptors were more strongly expressed in the cell proliferating zone (63-100%). In SCC, perlecan and KGF disappeared from carcinoma cells but emerged in the stromal space (65-100%), while VEGF, SHH, and VEGF receptors remained positive in SCC cells (0%). Immunofluorescence showed that the four growth factors were shown to be produced by three oral SCC cell lines and that their signals were partially overlapped with perlecan signals. The results indicate that perlecan and its binding growth factors are differentially expressed and function in specific manners before (dysplasia/CIS) and after (SCC) invasion of dysplasia/carcinoma cells.

Media Type:

Electronic Article

Year of Publication:

2016

Contained In:

Pathology, research and practice - Vol. 212, No. 5 (2016), p. 426-36

Language:

English

Contributors:

Hasegawa, Mayumi
Cheng, Jun
Maruyama, Satoshi
Yamazaki, Manabu
Abé, Tatsuya
Babkair, Hamzah
Saito, Chikara
Saku, Takashi

Urls:

Volltext

Keywords:

126469-10-1
143972-95-6
Carcinoma, Squamous Cell
Carcinoma in Situ
Carcinoma in situ
Cell Proliferation
Cell proliferating zone
EC 2.7.10.1
Epithelial Cells
Epithelial dysplasia
Epithelium
FGF7 protein, human
FLT1 protein, human
Fibroblast Growth Factor 7
Hedgehog Proteins
Heparan Sulfate Proteoglycans
Humans
Hyperplasia
Journal Article
Mouth Mucosa
Mouth Neoplasms
Oral squamous cell carcinoma
Perlecan
Perlecan-binding growth factors
Precancerous Conditions
SHH protein, human
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2

Notes:

Date Completed 23.03.2017

Date Revised 23.03.2017

published: Print-Electronic

Citation Status MEDLINE

Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Physical Description:

Online-Ressource

doi:

10.1016/j.prp.2016.02.016

PMID:

26965914

PPN (Catalogue-ID):

NLM258327480